Chemical Characterization and Antibacterial Activity of Phases Obtained from Extracts of Artemisia herba alba, Marrubium vulgare and Pinus pinaster

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The objective of this study is the phytochemical characterization and antibacterial activity of Artemisia herba alba, Marrubium vulgare and Pinus pinaster. The antibacterial potential of different fractions of ethyl acetate, diethyl ether and 1 butanol extracts of the three plants were tested on four strains of bacteria: Staphylococcus aureus, Escherichia coli, Proteus vulgaris and Pseudomonas aeruginosa. The results of phytochemical screening showed a rich and varied composition of secondary metabolites: flavonoids, tannins, terpenes, sterols, coumarins and saponins which have characterized all the crude extracts, while alkaloids particularize Pinus pinaster. This result was confirmed by analytical thin layer chromatography on some secondary metabolites such as flavonoids, tannins, terpenes and sterols of the three phases obtained after exhaustion of three plant extracts with organic solvents of increasing polarity. The TLC on polyamide gel revelation by NEU reagent, allowed to assume that the plants tested contain various flavonoids. The chromatographic analysis on silica plate has highlighted several fluorescences with the NEU reagent, reagent of Liebermann-Burchard and FeCl3 suggesting the presence of flavonoids, terpenes, sterols and tannins. Spectral analysis of methanol extracts advance the predominance of natural flavonoids: Flavonol, Flavone, Flavanone, Flavanol or Anthocyane. The antibacterial activity of different fractions performed on solid agar medium showed little or no effect; this implies that the antibacterial activity proved with crude extracts of these plants in previous studies is probably due to a synergistic action in chemical ingredients present in the extracts.


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